AIM: To investigate the effect of Dan-shao-hua-xian (DSHX) capsule on the expression of the family of microRNA-200 (miR-200s) in rat fibrotic livers. METHODS: Forty male Wistar rats weighing 180 g to 220 g were divided into 5 groups (control group, two model groups and two interference groups). The rats in model groups and interference groups were induced by hypodermic injection of CCl4 for 4 weeks and 8 weeks. The rats in interference groups were also treated with DSHX capsule (0.5 g/kg) once daily for 4 weeks and 8 weeks at the same time. The liver index and serum activity of ALT and AST were analyzed. The liver fibrosis was observed under microscope. Additionally, the expression of miR-200a, -200b, -200c, -141 and -429 was determined by quantitative real-time PCR. RESULTS: The liver index, and serum activity of ALT and AST in model groups and 4-week interference group were obviously higher than those in normal control group. The apparent liver fibrosis was observed in 8-week model group. The expression of miR-200a,-200b, -200c, -141 and -429 in the liver of 8-week model groups was obviously higher than that in control group. CONCLUSION: In the process of liver fibrosis induced by CCl4, the obvious changes of miR-200s may play an important role in the development of liver fibrosis. The miR-200s might be the potential target that DSHX capsule inhibits the process of liver fibrosis.