Effects of Kv1.5 on proliferation and apoptosis of rat PASMCs under hypoxia+hypercapnia condition and relationship with MAPK pathway
MA Ying-chun1, ZHENG Meng-xiao1, HUANG Lin-jing1, WANG Yuan-yuan3, YING Lei1, 2, WANG Wan-tie1, 2
1Department of Pathophysiology, School of Basic Medical Sciences, 2Institute of Ischemia/Reperfusion Injury, Wenzhou Medical University, Wenzhou 325035, China; 3Department of Pathology, Tongde Hospital of Zhejiang Province, Hangzhou 310012, China.
AIM：To investigate the effects of voltage-dependent K＋ channel 1.5 (Kv1.5) on the proliferation and apoptosis of rat pulmonary artery smooth muscle cells (PASMCs) under hypoxia+hypercapnia condition and the relationship with mitogen-activated protein kinase(MAPK) signal pathway. METHODS：The PASMCs isolated from the male SD rat were cultured under hypoxia+hypercapnia condition, and randomly divided into normal group (N group), hypoxia+hypercapnia group (HH group), hypoxia+hypercapnia+DMSO incubation group (HD group), hypoxia+hypercapnia+U0126 (an extracellular signal-regulated kinase 1/2 inhibitor) incubation group (HU group), hypoxia+hypercapnia+SB203580 (a p38 mitogen-activated protein kinase inhibitor) incubation group (HS group), and hypoxia+hypercapnia+anisomycin (an agonist of MAPK) incubation group (HA group). Cell Counting Kit-8 was used to detect the cell viability. The protein expression of Kv1.5, PCNA and Bax was detected by Western blotting. RESULTS：Compared with N group, the cell viability and PCNA protein expression in HH group and HD group were significantly raised (P<001), but Kv1.5 and Bax proteins were significantly decreased (P<0.01). No difference between HH group and HD group was observed (P>005). Compared with HD group, the cell viability and PCNA protein expression in HU group, HS group and HA group were decreased (P<0.05 or P<0.01), but Kv1.5 protein and Bax protein were raised (P<0.01), with the most significant changes in HA group. CONCLUSION：The regulation of Kv1.5 to the proliferation and apoptosis of PASMCs under hypoxia+hypercapnia condition might have a relationship with the activation of MAPK signal pathway.
马迎春,郑梦晓,黄林静,王园园,应磊,王万铁. Kv1.5对大鼠低氧高二氧化碳性PASMCs增殖、凋亡的影响及与MAPK通路的关系[J]. 中国病理生理杂志, 2014, 30(9): 1645-1650.
MA Ying-chun, ZHENG Meng-xiao, HUANG Lin-jing, WANG Yuan-yuan, YING Lei, WANG Wan-tie. Effects of Kv1.5 on proliferation and apoptosis of rat PASMCs under hypoxia+hypercapnia condition and relationship with MAPK pathway. Chin J Pathophysiol, 2014, 30(9): 1645-1650.
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