1. Research Center of Pathophysiology; 2. Department of Ophthalmology, The Second Affiliated Hospital, Fujian University of Traditional Chinese Medicine, Fuzhou 350003, China. E-mail: qihuang@netease.com
Abstract：AIM: To investigate the effect of isopsoralen(ISR) on the expression of estrogen receptor (ER) in human lens epithelial cells (HLECs). METHODS: HLECs were cultured and sub-cultured in vitro. The cultured HLECs pretreated with E2 or ISR were exposed to H2O2 at the concentration of 300 μmol/L. The expression of ERα and ERβ in HLECs was analyzed by flow cytometry. RESULTS: The expression of ERα and ERβ in H2O2 group was obviously decreased as compared to control group (P<0.01). The expression of ERα and ERβ in the cells treated with E2 and with ISR at the concentration of 10-5 mol/L, 10-6 mol/L or 10-7 mol/L plus H2O2 was obviously increased as compared to the cells treated with H2O2 only (P<0.01). A concentration-dependent effect of ISR was observed. CONCLUSION: H2O2 decreases the expression of ERα and ERβ in HLECs.E2 and ISR increase the expression of ERα and ERβ in HLECs treated with H2O2 in a concentration-dependent manner, which may account for their antioxidative effect.
Ogueta SB, Schwartz SD, Yamashita CK, et al. Estrogen receptor in the human eye:influence of gender and age on gene expression[J]. Invest Ophthalmol Vis Sci, 1999,40(9):1906-1911.
[6]
Cammarata PR, Chu S, Moor A, et al. Subcellular distribution of native estrogen receptor alpha and beta subtypes in cultured human lens epithelial cells[J]. Exp Eye Res, 2004, 78 (4):861-871.
[7]
Cammarata PR, Flynn J, Gottipati S, et al. Differential expression and comparative subcellular localization of estrogen receptor beta isoforms in virally transformed and normal cultured human lens epithelial cells[J]. Exp Eye Res,2005, 81(2):165-175.
[8]
Tamir S, Izrael S, Vaya J. The effect of oxidative stress on ERα and ERβ expression[J]. J Steroid Biochem Mol Biol, 2002,81(4-5):327-332.
[9]
Wang X, Simpkins JW, Dykens JA, et al. Oxidative damage to human lens epithelial cells in culture:estrogen protection of mitochondrial potential, ATP, and cell viability[J]. Invest Ophthalmol Vis Sci, 2003,44(5):2067-2075.
[10]
Moor AN, Flynn JM, Gottipati S. 17β-estradiol stimulates MAPK signaling pathway in human lens epithelial cell cultures preventing collapse of mitochondrial membrane potential during acute oxidative stress [J]. Mitochondrion, 2005, 5(4):235-247.
[11]
Moor AN, Gottipati S, Mallet RT, et al. A putative mitochondrial mechanism for antioxidative cytoprotection by 17 beta-estradiol[J]. Exp Eye Res, 2004,78(5): 933-944.
[12]
Flynn JM, Cammarata PR. Estradiol attenuates mitochondrial depolarization in polyol-stressed lens epithelial cells[J]. Mol Vis, 2006, 12(4):271-282.
[13]
Gottipati S, Cammarata PR. Mitochondrial superoxide dismutase activation with 17 beta-estradiol-treated human lens epithetlial cells[J].Mol Vis, 2008, 14(5):898-905.
Nagira K, Sasaoka T, Wada T,et al. Altered subcellular distribution of estrogen receptor is implicated in estradiol-induced dual regulation of insulin signaling in 3T3-L1 adipocytes[J]. Endocrinology, 2006,147(2):1020-1028.
