Abstract：AIM: To analyze the relationship between the expression of signal transducer and activator of transcription3 (STAT3) and suppressor of cytokine signaling3 (SOCS3),and endometriosis. METHODS: Ectopic (ectopic group, n=30) and eutopic endometrial tissues (eutopic group, n=30) were obtained from the women with histopathologically confirmed endometriosis. Normal endometrial tissues (control group, n=25) were collected from the women without endometriosis. Western blotting analysis was used to assess the protein expression of SOCS3, total STAT3 and its activated form phosphorylated STAT3. RESULTS: No significant difference of total STAT3 protein level among the 3 groups was observed, while the level of p-STAT3/STAT3 (representing the activating extent) was significantly higher in ectopic group than that in control group and eutopic group. Expression of SOCS3 protein was opposite to the activation level of STAT3. Negative correlation was showed between the activation level of STAT3 and the expression of SOCS3 protein (r=-0.499, P<0.01) in the patients with endometriosis. CONCLUSION: Activation of STAT3 is higher in the ectopic endometrium of endometriosis patients than that in the patients with eutopic endometrium and controls, which is negatively correlated with the expression of SOCS3 protein, indicating that dysregulation of STAT3 activation and SOCS3 expression may participate in the pathogenesis and progression of endometriosis.
Guo SW. Recurrence of endometriosis and its control[J]. Hum Reprod Update,2009,15 (4):441-461.
[4]
Niwa Y, Kanda H, Shikauchi Y, et al. Methylation silencing of SOCS3 promotes cell growth and migration by enhancing JAK/STAT and FAK signalings in human hepatocellular carcinoma [J]. Oncogene, 2005, 24 (42):6406-6417.
Dimitriadis E, Stoikos C, Tan YL, et al. Interleukin 11 signaling components signal transducer and activator of transcription 3 (STAT3) and suppressor of cytokine signaling 3 (SOCS3) regulate human endometrial stromal cell differentiation [J]. Endocrinology, 2006, 147 (8):3809-3817.
[7]
Klemmt PA, Carver JG, Kennedy SH, et al. Stromal cells from endometriotic lesions and endometrium from women with endometriosis have reduced decidualization capacity [J]. Fertil Steril, 2006, 85 (3):564-572.
[8]
Puhr M, Santer FR, Neuwirt H, et al. SOCS3 antagonises the proliferative and migratory effects of fibroblast growth factor-2 in prostate cancer by inhibition of p44/p42 MAPK signalling [J]. Endocr Relat Cancer, 2010, 17 (2):525-538.
