Abstract：AIM:To observe the dynamic changes of zinc finger E-box binding homeobox 1(ZEB1) and zinc finger E-box binding homeobox 2(ZEB2) during the development of liver fibrosis induced by chronic liver injury in rats. METHODS:Forty male Wistar rats weighing 180~220 g were divided intonormal control group and 4 model groups. The rats in model groups were induced by hypodermic injection of CCl4 at a dose of 3 mL/kg and once per 3 days for 2 weeks, 4 weeks, 6 weeks and 8 weeks, respectively. The liver index and serum levels of alanine aminotransferase(ALT) and aspartate aminotransferase(AST) were analyzed. The liver fibrosis was observed under microscope. Additionally, the expression of ZEB1, ZEB2, E-cadherin and α-smooth muscle actin(α-SMA) SMA at protein level was determined by immunohistochemistry. The mRNA expression of ZEB1 and ZEB2 was detected by real-time RT-PCR. RESULTS:The liver index and the serum levels of ALT and AST in the 4 model groups were obviously higher than those in normal control group. The apparent liver fibrosis was observed in 8-week model group. The protein levels of α-SMA(37.84±7.04), ZEB1(46.42±14.36) and ZEB2(57.71±13.32), and mRNA expression of ZEB1(189.00±47.39) and ZEB2(277.28±48.55) in the livers of 8-week model rats were obviously higher than those in the control rats(P<0.01). The protein level of E-cadherin in the liver of 8-week model rats was obviously lower than that in the control rats(P<0.01). CONCLUSION:In the process of liver fibrosis induced by CCl4, the obvious changes of ZEB1/ZEB2 may play an important role in chronic liver injury and liver fibrosis.
韩冰, 谢汝佳, 洪琴, 张成俊, 杨勤, 程明亮. 肝纤维化病理过程中ZEB1和ZEB2的动态表达变化及意义[J]. 中国病理生理杂志, 2012, 28(9): 1639-1643.
HAN Bing, XIE Ru-jia, HONG Qin, ZHANG Cheng-jun, YANG Qin, CHENG Ming-liang. Dynamic changes of ZEB1 and ZEB2 during development of liver fibrosis induced by chronic liver injury in rats. Chin J Pathophysiol, 2012, 28(9): 1639-1643.
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