Inhibitory effect of Ophiopogon japonicus on rat cardiac fibroblasts
WANG Guo-qin1,LI Rui-man2,JIN Wei3,SONG Guo-liang3,CHEN Qin3,PEI Zhao-hui3
1Department of Traditional Chinese Medicine, 3Department of Cardiology, Nanchang Third Municipal Hospital, Nanchang 330009, China; 2Department of Obstetrics and Gynaecology, the First Affiliated Hospital, Jinan University, Guangzhou 510632, China.
AIM:To investigate the inhibitory effect of Ophiopogon japonicus on rat cardiac fibroblast (CFs) and the underlying mechanism. METHODS:Cultured CFs from Sprague-Dawley (SD) rats were randomized into 4 groups: control group (normal rat cardiac fibroblasts), Ophiopogon japonicus of 10 μg/L group, Ophiopogon japonicus of 20 μg/L group and Ophiopogon japonicus of 30 μg/L group. Cell vitality, [3H]-proline incorporation, and the protein expression of TGF-β1, p-Smad2/3 and total Smad2/3 in CFs were determined. RESULTS:Compared with control, the cell vitality, [3H]-proline incorporation, and the protein expression of TGF-β1, p-Smad2/3 and total Smad2/3 were significantly decreased in Ophiopogon japonicus of 10 μg/L group. Compared with Ophiopogon japonicus of 10 μg/L group, the cell vitality, [3H]-proline incorporation, and the protein expression of TGF-β1, p-Smad2/3 and total Smad2/3 were significantly decreased in Ophiopogon japonicus of 20 μg/L group. Compared with Ophiopogon japonicus of 20 μg/L group, the cell vitality,[3H]-proline incorporation, and the protein expression of TGF-β1, p-Smad2/3 and total Smad2/3 were significantly decreased in Ophiopogon japonicus of 30 μg/L group. CONCLUSION:Ophiopogon japonicus may inhibit CFs. These actions are related to the changes of [3H]-proline incorporation, and the protein expression of TGF-β1, p-Smad2/3 and total Smad2/3.