Comparison of two methods for culturing neonatal rat cardiac fibroblasts
SUN Gang, XUE Rui-cong, LIU Chen, DONG Yu-gang
1Department of Cardiology, The First Affiliated Hospital, 2Key Laboratory of Assisted Circulation, Ministry of Health, Sun Yat-sen University, Guangzhou 510080, China.
AIM:To establish and compare the methods for culturing neonatal rat cardiac fibroblasts culture. METHODS:Neonatal rat hearts were isolated by collagenase+trypsin or trypsin digestion. The cardiomyocytes and cardiac fibroblasts were isolated by different attachment techniques. The cellular morphology, purity and reactions to the reagent were tested. RESULTS:For morphology and purity, no difference between the 2 methods was observed, though more myocytes were harvested by the method of collagenase+trypsin digestion. For the cellular responses to reagent, the cardiac fibroblasts harvested with trypsin digestion had more potent proliferative ability than those with collagenase+trypsin digestion. CONCLUSION:There is no difference of cellular morphology and purity in the cardiac fibroblasts isolated by collagenase+trypsin digestion and trypsin digestion, but the fibroblasts with trypsin digestion have more potent proliferative ability.
孙刚,薛睿聪,刘晨,董吁钢. 新生大鼠心肌成纤维细胞培养方法的比较[J]. 中国病理生理杂志, 2014, 30(2): 380-384.
SUN Gang, XUE Rui-cong, LIU Chen, DONG Yu-gang. Comparison of two methods for culturing neonatal rat cardiac fibroblasts. Chin J Pathophysiol, 2014, 30(2): 380-384.