Thalidomide inhibits TGF-β1-induced activation of CTGF gene promoter in HELF cell line
WANG Da-an1,2, LIN Yi-xin3, WANG Zheng3, SANG Chao-lei3, LU Da-xiang1, 2, WANG Hua-dong1, 2, HU Chao-feng1, 2, WEI Wei1, 2, JIANG Jian-wei4, FU Yong-mei1, 2, LI Hong-mei1,2
1Department of Pathophysiology, 2Key Laboratory of State Administration of Traditional Chinese Medicine, 3Grade 2010 Undergraduate Program for Stomatology, 4Department of Biochemistry, School of Medicine, Jinan University, Guangzhou 510632, China.
AIM: To investigate the effects of thalidomide (THD) on the activation of connective tissue growth factor (CTGF) gene promoter induced by transforming growth factor β1 (TGF-β1) in human embryonic lung fibroblasts (HELF). METHODS: DNA sequence of CTGF gene promoter was cloned into luciferase reporter gene vector to construct the recombinant eukaryotic expression vector pGL3-CTGFP, and the recombinant vector was transfected into HELF cell line. The effects of TGF-β1 and THD on the activation of CTGF gene promoter were detected by dual-luciferase analysis. RESULTS: TGF-β1 increased the reporter gene activity dose-dependently (P<0.05), with a plateau at 5 μg/L being 2.16 folds as high as the control. TGF-β1-induced increase in the reporter gene activity was also time-dependent (P<0.05). After exposure to TGF-β1 (5 μg/L), the level of luciferase activity reached its peak at 12 h and was 2.52 folds as high as the control. THD significantly inhibited TGF-β1-induced increase in the reporter gene activity in a dose-dependent manner, but its basal activity was not changed. CONCLUSION: TGF-β1 stimulates the transcriptional activity of CTGF gene promoter in HELF cells in a dose- and time-dependent manner, while THD may inhibit the effects dose-dependently.
王达安,林逸心,王峥,桑朝磊,陆大祥,王华东,胡巢凤,魏伟,蒋建伟,付咏梅,李红梅. 沙利度胺抑制TGF-β1诱导的HELF细胞中结缔组织生长因子基因启动子的激活*[J]. 中国病理生理杂志, 2014, 30(4): 693-697.
WANG Da-an, LIN Yi-xin, WANG Zheng, SANG Chao-lei, LU Da-xiang, WANG Hua-dong, HU Chao-feng, WEI Wei, JIANG Jian-wei, FU Yong-mei, LI Hong-mei. Thalidomide inhibits TGF-β1-induced activation of CTGF gene promoter in HELF cell line. Chin J Pathophysiol, 2014, 30(4): 693-697.
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