梓醇促进大鼠骨髓间充质干细胞增殖过程中Wnt信号通路的变化

doi:10.3969/j.issn.1000-4718.2014.09.019

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摘要

目的：观察梓醇促进大鼠骨髓间充质干细胞（bone marrow mesenchymal stem cells, BMSCs）增殖过程中Wnt信号通路的变化。方法：（1）采用机械分离及差速贴壁法分离培养SD大鼠BMSCs，将细胞分为空白对照组与梓醇（1.0 mg/L）处理组，流式细胞术检测各组细胞细胞周期分布情况，计算其增殖指数。（2）实时荧光定量PCR法检测各组细胞中Wnt3a、Wnt5a、Wnt11及β-catenin mRNA的表达水平；Western blotting技术检测各组细胞β-catenin蛋白的表达变化。结果：（1）空白对照组与梓醇处理组BMSCs增殖指数分别为8.90%±0.46%和17.93%±1.68%（P<0.01）。(2）与空白对照组相比，梓醇处理组Wnt5a、Wnt11、β-catenin mRNA及β-catenin蛋白表达均明显提高，但Wnt3a mRNA表达无显著变化。结论：梓醇在促进BMSCs增殖过程中可同时激活经典与非经典Wnt信号通路。

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	傅淑平
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关键词 ：梓醇, 骨髓间充质干细胞, 细胞增殖, Wnt信号通路

Abstract：

AIM：To investigate the changes of Wnt signaling pathway in catalpol-induced proliferation of rat bone marrow mesenchymal stem cells (BMSCs). METHODS：The BMSCs were isolated from SD rats, purified by differential time adherent method and divided into control group and catalpol (1.0 mg/L) group. Flow cytometry was used to detect the proliferation index of BMSCs. The mRNA levels of Wnt3a, Wnt5a, Wnt11 and β-catenin was evaluated by real-time PCR. In addition, the protein expression level of β-catenin was determined by Western blotting. RESULTS：Prolife-ration index was increased from 8.90%±0.46% to 17.93%±1.68% after treatment with catalpol (P<0.01). Compared with control group, the mRNA expression of Wnt5a, Wnt11 and β-catenin was all increased with catalpol treatment. No difference of Wnt3a mRNA expression between control group and catalpol group was observed. Meanwhile, the protein expression of β-catenin was increased in catalpol group compared with control group. CONCLUSION：Catalpol promotes BMSCs going into the cell cycle. Classical and non-classical Wnt signaling pathways are activated in this process.

Key words： Catalpol Bone marrow mesenchymal stem cells Cell proliferation Wnt signaling pathway

收稿日期: 2014-03-14 出版日期: 2014-09-15

中图分类号:

R363

基金资助:

江苏省自然科学基金资助项目（No.BK20130956）;国家自然科学基金资助项目（No.81173619）;江苏省高校自然科学基金资助项目(No.12KJB360005);南京中医药大学中医学优势学科开放课题（No.YS2012ZYX410）；南京中医药大学青年自然科学基金（No.12XZR17）

通讯作者: 傅淑平 E-mail: tzrh@jnu.edu.cn; shuping.fu@gmail.com

引用本文:

傅淑平,杨丽,洪浩,偶晨,张荣华. 梓醇促进大鼠骨髓间充质干细胞增殖过程中Wnt信号通路的变化[J]. 中国病理生理杂志, 2014, 30(9): 1656-1660. FU Shu-ping, YANG Li, HONG Hao, OU Chen, ZHANG Rong-hua. Wnt signaling pathway is involved in catalpol-induced proliferation of rat bone marrow mesenchymal stem cells. Chin J Pathophysiol, 2014, 30(9): 1656-1660.

链接本文:

http://www.cjpp.net/CN/10.3969/j.issn.1000-4718.2014.09.019 或 http://www.cjpp.net/CN/Y2014/V30/I9/1656

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