Wnt signaling pathway is involved in catalpol-induced proliferation of rat bone marrow mesenchymal stem cells
FU Shu-ping1, YANG Li2, HONG Hao1, OU Chen1, ZHANG Rong-hua2
1Key Laboratory of Acupuncture and Medicine Research of Minister of Education, Nanjing University of Chinese Medicine, Nanjing 210023, China; 2College of Pharmacy, Jinan University, Guangzhou 510632, China.
AIM:To investigate the changes of Wnt signaling pathway in catalpol-induced proliferation of rat bone marrow mesenchymal stem cells (BMSCs). METHODS:The BMSCs were isolated from SD rats, purified by differential time adherent method and divided into control group and catalpol (1.0 mg/L) group. Flow cytometry was used to detect the proliferation index of BMSCs. The mRNA levels of Wnt3a, Wnt5a, Wnt11 and β-catenin was evaluated by real-time PCR. In addition, the protein expression level of β-catenin was determined by Western blotting. RESULTS:Prolife-ration index was increased from 8.90%±0.46% to 17.93%±1.68% after treatment with catalpol (P<0.01). Compared with control group, the mRNA expression of Wnt5a, Wnt11 and β-catenin was all increased with catalpol treatment. No difference of Wnt3a mRNA expression between control group and catalpol group was observed. Meanwhile, the protein expression of β-catenin was increased in catalpol group compared with control group. CONCLUSION:Catalpol promotes BMSCs going into the cell cycle. Classical and non-classical Wnt signaling pathways are activated in this process.
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FU Shu-ping, YANG Li, HONG Hao, OU Chen, ZHANG Rong-hua. Wnt signaling pathway is involved in catalpol-induced proliferation of rat bone marrow mesenchymal stem cells. Chin J Pathophysiol, 2014, 30(9): 1656-1660.
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