Protective effect of capsaicin on lipopolysaccharide-induced activation of vascular endothelial cells
LU Yang2, ZHAO Guang-ju1, HONG Guang-liang1, QIU Qiao-meng1, LI Dong1, LU Zhong-qiu1
1Emergency Department, The First Affiliated Hospital, 2Rheumatology Department,Wenzhou Third Clinical College, Wenzhou Medical University, Wenzhou 325000, China
AIM:To investigate the effect of capsaicin on lipopolysaccharide (LPS)-induced activation of cultured endothelial cells of mouse aorta in vitro. METHODS:The endothelial cells were isolated from mouse aorta and cultured in vitro, and the specific cell markers of the cells were identified by immunofluorescence staining. The cells were stimulated with LPS (100 μg/L) combined with or without capsaicin, and the cells and supernatant were collected at 12 h, 24 h and 48 h. The levels of soluble intercellular adhesion molecule 1 (sICAM-1), soluble vascular cell adhesion molecule 1 (sVCAM-1) and soluble P-selectin (sP-selectin) in the supernatant were measured by ELISA. The levels of nuclear NF-κB p65 and cytopasmic p-IκBα and IκBα were detected by Western blotting. RESULTS:Compared with control group, the levels of sP-selectin, sICAM-1 and sVCAM-1 in LPS group were significantly increased (P<0.05), and LPS promoted the expression of sICAM-1 and sVCAM-1 in a time-dependent manner. Compared with LPS group at the same time point, capsaicin inhibited the expression of sP-selectin, sICAM-1 and sVCAM-1 in a dose-dependent manner. Compared with control group, the protein levels of NF-κB p65 and p-IκBα in LPS group at 24 h were significantly increased (P<0.05), while the protein level of IκBα in LPS group at 24 h were significantly decreased (P<0.05). Compared with LPS group, capsaicin decreased the protein levels of NF-κB p65 and p-IκBα and increased the protein level of IκBα in a dose-dependent manner. CONCLUSION: Capsaicin has a protective effect on LPS-induced vascular endothelial cell activation, which potentially contributes to the suppression of IκBα degradation and NF-κB p65 nuclear translocation.
卢阳，赵光举，洪广亮，邱俏檬，李冬，卢中秋. 辣椒素对脂多糖诱导血管内皮细胞活化的影响及机制[J]. 中国病理生理杂志, 2014, 30(10): 1748-1752.
LU Yang, ZHAO Guang-ju, HONG Guang-liang, QIU Qiao-meng, LI Dong, LU Zhong-qiu. Protective effect of capsaicin on lipopolysaccharide-induced activation of vascular endothelial cells. Chin J Pathophysiol, 2014, 30(10): 1748-1752.
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