扶肾降浊方含药血清对肾小管间质损害大鼠成纤维细胞TGF-β1/Smads信号转导通路的影响

doi:10.3969/j.issn.1000-4718.2014.11.021

摘要
图/表
参考文献(0)
相关文章 (1)

全文: PDF (1458 KB) HTML (1 KB)
输出: BibTeX | EndNote (RIS)

摘要

目的:探讨扶肾降浊方对系膜增生性肾小球肾炎（MsPGN）大鼠肾小管间质损害的疗效机制。方法:采用扶肾降浊方水溶液灌胃干预Wistar大鼠常规制备含药血清；在MsPGN动物模型基础上，延长造模时间至20周，使其自然发展为肾小管间质损害模型，体外培养12、16和20周模型大鼠间质成纤维细胞；采用real-time PCR和Western blotting法检测扶肾降浊方含药血清对病理状态间质成纤维细胞转化生长因子-β1（TGF-β1）、Smad3和Smad7 mRNA及蛋白表达的影响。结果:MsPGN大鼠间质成纤维细胞TGF-β1和Smad3 mRNA和蛋白表达上调，Smad7 mRNA和蛋白表达下调。扶肾降浊方含药血清随着给药周期的延长可部分逆转MsPGN间质损害造成的上述mRNA和蛋白表达异常。结论: 扶肾降浊方含药血清对MsPGN大鼠间质成纤维细胞保护作用可能与调节TGF-β1/Smads信号转导通路有关。

	服务

	把本文推荐给朋友
	加入我的书架
	加入引用管理器
	E-mail Alert
	RSS
	作者相关文章
	李春雨
	苏玮莲
	魏晓露
	李国霞

关键词 ：扶肾降浊方, 肾间质成纤维细胞, 血清药理法

Abstract：

AIM:To study the protective effects of Fushen Jiangzhuo formula (FSJZ) on renal interstitial fibroblasts in the rats with renal tubulointerstitial lesion. METHODS:The serum containing FSJZ and blank serum were prepared. The rat model of mesangial proliferative glomerulonephritis (MsPGN) was established, and the tubulointerstitial lesion model was developed by extending the time of the MsPGN model to 20 weeks. The renal interstitial fibroblasts from the model rats were cultured at 12, 16 and 20 weeks. The effect of FSJZ on the expression of transforming growth factor beta 1 (TGF-β1), Smad3 and Smad7 at mRNA and protein levels in the pathological renal interstitial fibroblasts was determined. RESULTS:The TGF-β1/Smads signal transduction pathway changed in pathological renal interstitial fibroblasts. The renal tubulointerstitial lesion significantly up-regulated the expression of TGF-β1 and Smad3, and down-regulated the expression of Smad7. The FSJZ-containing serum significantly down-regulated the expression of TGF-β1 and Smad3, and up-regulated the expression of Smad7, thus antagonizing the effects caused by renal tubulointerstitial lesion. CONCLUSION: FSJZ-containing serum has protective effects on renal interstitial fibroblasts. The mechanism may be related to the regulatory effects of FSJZ on TGF-β1/Smads signal transduction pathway.

Key words： Fushen Jiangzhuo formula Renal interstitial fibroblast Serologic pharmacological test

收稿日期: 2014-05-15 出版日期: 2014-11-15

中图分类号:	R363
	R259

基金资助:

天津市自然科学基金资助项目（No. 11JCYBJC12800）

通讯作者: 李国霞 E-mail: liguoxia96@163.com

引用本文:

李春雨，苏玮莲，魏晓露，李国霞. 扶肾降浊方含药血清对肾小管间质损害大鼠成纤维细胞TGF-β1/Smads信号转导通路的影响[J]. 中国病理生理杂志, 2014, 30(11): 2043-2047. LI Chun-yu, SU Wei-lian, WEI Xiao-lu, LI Guo-xia. Effect of serum containing Fushen Jiangzhuo formula on TGF-β1/Smads signal transduction pathway in renal interstitial fibroblast of renal tubulointerstitial lesion rat model. Chin J Pathophysiol, 2014, 30(11): 2043-2047.

链接本文:

http://www.cjpp.net/CN/10.3969/j.issn.1000-4718.2014.11.021 或 http://www.cjpp.net/CN/Y2014/V30/I11/2043

［1］	刘春莹,陈磊鑫,冷伟,等. 复方肾复片对MsPGN大鼠肾小管间质损害的影响［J］.现代中医药, 2013, 33(3):89-92.
［2］	Huang C, Shen S, Ma Q, et al. Blockade of KCa3.1 ameliorates renal fibrosis through the TGF-β1/Smad pathway in diabetic mice［J］. Diabetes, 2013, 62(8): 2923-2934.
［3］	李国霞,黄文政,何永生. 扶肾降浊法治疗慢性肾功能衰竭80例临床观察［J］.山东中医杂志, 2005, 24(4):207-209.
［4］	李国霞,黄文政,何永生. 扶肾降浊法对肾功能衰竭大鼠常规指标的影响［J］.天津中医药, 2005, 22(2):108-110.
［5］	刘震,周树录,谭建三，等. 大鼠系膜增生型肾小球肾炎模型的改进［J］.华西医科大学学报, 1996, 27(2):182-184.
［6］	李国霞,黄文政,朱小棣. 系膜增生性肾小球肾炎肾小管间质损害模型的建立及肾疏宁的保护作用［J］.天津中医药, 2007, 24(4):325-327.
［7］	高巧艳,李明才,李燕子,等. IL-33/ST2信号通路在纤维化疾病中的作用［J］.中国病理生理杂志, 2013, 29(9):1712-1717.
［8］	宋囡,何文智,王智民,等. 左、右归丸及其拆方对骨髓间充质干细胞成骨分化的影响［J］.中国病理生理杂志, 2013, 29(7):1268-1274.
［9］	张晨月,张舒,吴莹,等. 犀角地黄汤合银翘散对流感病毒感染的小鼠肺组织及大鼠肺微血管内皮细胞ICAM-1和VCAM-1表达的影响［J］.中国病理生理杂志, 2013, 29(9):1685-1690.
［10］	Ge YZ, Wu R, Lu TZ, et al. Combined effects of TGFB1+869 T/C and +915 G/C polymorphisms on acute rejection risk in solid organ transplant recipients: a systematic review and meta-analysis［J］. PLoS One, 2014, 9(4): e93938.
［11］	Zhao TT, Zhang HJ, Lu XG,et al.Chaihuang-Yishen granule inhibits diabetic kidney disease in rats through blocking TGF-β/Smad3 signaling［J］. PLoS One, 2014, 9(3):e90807.
［12］	Ryoo IG, Ha H, Kwak MK. Inhibitory role of the KEAP1-NRF2 pathway in TGFβ1-stimulated renal epithelial transition to fibroblastic cells: a modulatory effect on SMAD signaling ［J］. PLoS One, 2014, 9(4):e93265.
［13］	Pan MM, Zhang MH, Ni HF, et al. Inhibition of TGF-β1/Smad signal pathway is involved in the effect of Cordyceps sinensisagainst renal fibrosis in 5/6 nephrectomy rats［J］. Food Chem Toxicol, 2013, 58(1): 487-494.