自噬对HIV-1 gp120V3环介导的海马神经元L型钙离子通道电流的影响

doi:10.3969/j.issn.1000-4718.2014.11.002

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摘要

目的:观察哺乳动物雷帕雷素靶蛋白（mTOR）依赖性自噬信号转导通路对HIV-1 gp120V3环介导的海马神经元L-型钙离子通道电流的影响。方法：取出生1 d内的乳鼠海马神经元原代培养7 d后用于实验。实验分为2个平行实验组，即空白对照组、gp120V3组、自噬阻断剂3-甲基腺嘌呤（3-MA）组和gp120V3+3-MA组；空白对照组、gp120V3组、自噬激动剂雷帕雷素（rapamycin)组和gp120V3+rapamycin组。以膜片钳技术记录海马神经元的L型钙离子通道电流。结果：gp120V3组及3-MA组与空白对照组比较，L型钙离子通道电流密度增大（P<0.05）；gp120V3+3-MA组与gp120V3组比较，L-型钙离子通道电流密度增大（P<0.05）；rapamycin组与空白对照组比较，L型钙离子通道电流密度减小（P<0.05）；gp120V3+rapamycin组与gp120V3组比较，L型钙离子通道电流密度减小（P<0.05）。结论：从电生理角度证明，自噬可能参与了HIV-1gp120V3环介导的海马神经元L型钙离子通道电流的改变。

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	汪军兵
	林丽清
	江明亮
	陆大祥
	陈桂玲
	行妍妍
	董军

关键词 ：自噬, HIV-1 gp120V3, 海马神经元, L型钙离子通道

Abstract：

AIM: To observe the influence of autophagy on L-type calcium channel current induced by gp120V3 loop in hippocampal neurons.METHODS: Hippocampal neurons were exteriorized from the newborn rats within 1 d and primarily cultured for 7 d. The neurons were divided into 2 parallel groups, namely control group, gp120V3 group, autophagy inhibitor 3-MA group, gp120V3 + 3-MA group; control group, gp120V3 group, autophagy activator rapamycin group, gp120V3 + rapamycin group. Whole-cell pathch-clamp was used to record L-type calcium channel current. RESULTS: Compared with control group, the density of L-type calcium channel current increased in gp120V3 group and 3-MA group(P<0.05). Compared with gp120V3 group, the density of L-type calcium channel current increased in gp120V3 + 3-MA group(P<0.05). Compared with control group, the density of L-type calcium channel current decreased in rapamycin group(P<0.05). Compared with gp120V3 group, the density of L-type calcium channel current increased in gp120V3 + rapamycin group(P<0.05).CONCLUSION: Autophagy may be involved in the gp120V3 loop-mediated L-type calcium channels in hippocampus neurons.

Key words： Autophagy HIV-1 gp120V3 Hippocampus neurons L-type calcium channel

收稿日期: 2014-05-30 出版日期: 2014-11-15

中图分类号:

R338.8

基金资助:

国家自然科学基金资助项目（No. 8117134； No. 81471235）; 广东省自然科学基金资助项目（No. 9151040701000）；广东省科技计划资助项目（No. 2010B030700016）；广州市科技计划资助项目（No. 2010Y1-C291）

通讯作者: 汪军兵 E-mail: dongjunbox@163.com

引用本文:

汪军兵，林丽清，江明亮，陆大祥，陈桂玲，行妍妍，董军. 自噬对HIV-1 gp120V3环介导的海马神经元L型钙离子通道电流的影响[J]. 中国病理生理杂志, 2014, 30(11): 1929-1933. WANG Jun-bing, LIN Li-qing, JIANG Ming-liang, LU Da-xiang, CHEN Gui-Ling, XING Yan-yan, DONG Jun. Effect of autophagy on L-type calcium channel current induced by gp120V3 in hippocampus neurons. Chin J Pathophysiol, 2014, 30(11): 1929-1933.

链接本文:

http://www.cjpp.net/CN/10.3969/j.issn.1000-4718.2014.11.002 或 http://www.cjpp.net/CN/Y2014/V30/I11/1929

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