心包腔注入炎性渗出液诱导大鼠三叉神经脊束核神经元c-Fos表达

doi:10.3969/j.issn.1000-4718.2014.11.001

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摘要

目的:本文旨在阐明从大鼠心包注入炎性渗出液(IES)与大鼠三叉神经脊束核（STT）神经元激活的相关性。
方法: 成年雄性Wistar大鼠用氨基甲酸乙酯麻醉后随机分为3组：从心包腔注入炎性渗出液为IES组；生理盐水组（saline 组）大鼠心包腔注入等量的生理盐水，其余手术过程同IES组；假手术组（sham 组）大鼠心包腔内既不注射IES也不注射生理盐水，手术程序同IES组。运用免疫组化方法，以神经元活化为线索，观察大鼠脊髓和脑干神经元c-Fos表达，特别关注大鼠脑干三叉神经感觉核复合体神经元c-Fos的表达，包括三叉神经感觉主核腹外侧部分（Pr5VL）以及三叉神经脊束核口部（Sp5O）、极间部（Sp5I）和尾部（Sp5C）。
结果: 同saline 组和sham 组相比，IES组大鼠C2段脊髓背角I-V层c-Fos阳性神经元数量显着增加（P＜0.05），三叉神经脊束核Sp5C、Sp5O、Sp5I及Pr5VL腹侧边c-Fos阳性神经元数量也明显增加（P＜0.05），呈圆锥体状的三叉神经感觉核复合体c-Fos阳性神经元的数量自前囟到对耳线逐渐增加，c-Fos阳性神经元的数量右侧少于左侧（P＜0.05）。Sp5C腹侧边c-Fos阳性神经元主要集中在胶质细胞层，大细胞层较少（P＜0.05）。用育亨宾（1 mg/kg， ip）和纳络酮（3 mg/kg， ip）预处理20 min，IES组大鼠Sp5C腹侧边c-Fos阳性神经元的表达未受影响。
结论: 向心包腔注入致痛物质模拟大鼠心肌缺血心绞痛，可以活化三叉神经脊束核神经元。这为临床上部分心肌缺血患者颅面部疼痛是其唯一的症状提供了实验依据。

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	王海华
	姜玉新
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	周萍萍
	包鹏举
	曾瑾
	王海珍

关键词 ：炎性渗出液, 心包内, 颅面部痛, 心肌缺血, 三叉神经脊束核神经元

Abstract：

AIM: To identify the correlation between intrapericardial injection of allogeneic chemicals and activation of the rat spinal trigeminal tract (STT) neurons.METHODS: Adult male Wistar rats were anesthetized with urethane and divided into 3 groups: inflammatory exudate solution (IES) group, receiving intrapericardial infusion of IES; negative control (saline) group, receiving intrapericardial infusion of saline; and sham group, undergoing the same surgical procedure without intrapericardial infusion. The expression of c-Fos in the neurons of the cervical spinal cord and brain stem, particularly in the region of the trigeminal sensory nuclear complex, including the principal sensory trigeminal nucleus (Pr5VL), subnucleus oralis (Sp5O), subnucleus interpolaris (Sp5I) and subnucleus caudalis (Sp5C), was analyzed by immunohistochemistry as an index of neuronal activation. RESULTS: Compared with saline group and sham group, the number of c-Fos-positive neurons in IES group increased significantly (P＜0.05). c-Fos-positive neurons were mainly found in the dorsal horns of laminae I-V at C2, as well as at the rostral edge of the Pr5VL, Sp5O, Sp5I, and Sp5C. The number of c-Fos-positive neurons gradually increased from bregma to interaural line at the pyramidal decussation. Interestingly, fewer c-Fos-positive neurons were observed on the right side than that on the left (P＜0.05).CONCLUSION: Intrapericardial injection of algogenic chemicals activates STT neurons, possibly underlying the phenomenon whereby cardiac ischemia causes craniofacial pain.

Key words： Inflammatory exudate solution intrapericardial Craniofacial pain Myocardial ischemia Spinal trigeminal tract neurons

收稿日期: 2014-04-21 出版日期: 2014-11-15

中图分类号:

R363

基金资助:

国家自然科学基金资助项目 (No. 81172790)；安徽省高校省级科学研究重点项目（No. KJ2013A251）；皖南医学院重点科研项目培育基金资助项目（No. WK2012Z01）；安徽省2012年省级教学研究重点项目（No. 2012jyxm313）

通讯作者: 王海华 E-mail: wanghaihua9972@sina.com

引用本文:

王海华，姜玉新, 王静,周萍萍，包鹏举，曾瑾，王海珍. 心包腔注入炎性渗出液诱导大鼠三叉神经脊束核神经元c-Fos表达[J]. 中国病理生理杂志, 2014, 30(11): 1921-1928. WANG Hai-hua, JIANG Yu-xin, WANG Jing, ZHOU Ping-ping, BAO Peng-ju, ZENG Jin, WANG Hai-zhen. c-Fos expression in spinal trigeminal tract neurons of rats induced by intrapericardiac injection of algogenic chemicals. Chin J Pathophysiol, 2014, 30(11): 1921-1928.

链接本文:

http://www.cjpp.net/CN/10.3969/j.issn.1000-4718.2014.11.001 或 http://www.cjpp.net/CN/Y2014/V30/I11/1921

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