IL-1β stimulated neuron activation via PI3K/Akt/mTOR pathway
GAN Na1,2, YIN Fei2, PENG Jing2, WU Li-wen2, HE Fang2, CHEN Chen2
1. Department of Clinical Medicine, Guangxi Medical College, Nanning 530021, China;
2. Department of Pediatrics, Xiang-ya Hospital, Central South University, Changsha 410008, China
AIM: To study the effect of interleukin-1β (IL-1β) on neuron activation during the process of medial temporal lobe epilepsy (MTLE).METHODS: IL-1β, rapamycin [an inhibitor of mammalian target of rapamycin (mTOR)]and lentiviral transfection to knockdown PI3K-p85 were used to pre-treat the neurons. The protein levels of PI3K-p85, p-Akt, p-p70S6K and MAP2 were detected and the relationship among the tested cytokines was analyzed. The neuron endocytosis was observed in each group. RESULTS: IL-1β increased the protein levels of PI3K-p85, p-Akt and p-p70S6K, up-regulated the expression of PI3K-p85 binding with IL-1RI in the neurons, and increased the neuron endocytosis compared with control group (P<0.05). These processes were inhibited by rapamycin and silence of PI3K-p85 (P<0.05). Inhibition of the PI3K-p85 binding to IL-1RI decreased the protein levels of p-Akt, p-p70S6K and MAP2 which were increased by IL-1β stimulation (P<0.05). CONCLUSION: IL-1β activates PI3K-p85 by binding with IL-1RI to promote the activation and proliferation of neuron synapses via PI3K/Akt/mTOR signaling pathway, which might be one of the mechanisms in MTLE chronic progress.
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