Effect of Toll like-receptor 4 on proliferation of cervical cancer cells by NF-κB signaling pathway
YAN Wei, XU Fang-gen, LIU An-wen, CAI Jing, WANG Shuo
1. Department of Oncology, The Second Affiliated Hospital of Nanchang University, Nanchang 330006, China;
2. Department of Digestive Medicine, The Second Affiliated Hospital of Nanchang University, Nanchang 330006, China
AIM: To investigate whether specific small interfering RNA (siRNA)targeting Toll-like receptor 4(TLR4)gene inhibits the proliferation of cervical cancer cell line HeLa through NF-κB signaling pathway and down-regulation of Bcl-2 expression. METHODS: Three specific TLR4 siRNAs and 1 negative siRNA were transfected respectively into HeLa cells. The expression of TLR4 at mRNA and protein levels was measured by reverse transcription-polymerase chain reaction (RT-PCR)and Western blotting, respectively. The protein expression of p65 and Bcl-2 was detected by Western blotting. The cell proliferation was determined by MTT assay and plate colony formation assay. The apoptotic rate of the cells and the cell cycle were analyzed by flow cytometry. RESULTS: In comparison with the other 2 kinds of TLR4 siRNAs, TLR4-siRNA-003 demonstrated the strongest silencing effect on TLR4 gene expression in the HeLa cells at 48 h after transfection. The expression of TLR4 at mRNA and protein levels was reduced by 62% and 89%, respectively. The protein levels of p65 and Bcl -2 significantly decreased. The growth rate of HeLa cells transfected with TLR4-siRNA-003 was significantly inhibited. Moreover, the cell apoptotic rate increased significantly and the cell cycle was arrested at G1 phase as the HeLa cells were transfected with TLR4-siRNA-003 for 48 h. CONCLUSION: Specific TLR4 siRNA effectively inhibits the expression of TLR4 and inhibits the proliferation of cervical cancer HeLa cells through NF-κB signaling pathway and down-regulation of Bcl-2 expression, indicating that TLR4 may be a new target for the treatment of cervical cancer.
颜伟, 许芳根, 刘安文, 蔡婧, 王硕. TLR4通过NF-κB信号途径对宫颈癌细胞增殖的影响[J]. 中国病理生理杂志, 2015, 31(2): 301-307.
YAN Wei, XU Fang-gen, LIU An-wen, CAI Jing, WANG Shuo. Effect of Toll like-receptor 4 on proliferation of cervical cancer cells by NF-κB signaling pathway. Chin J Pathophysiol, 2015, 31(2): 301-307.
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