Effect of microRNA-155 on regulation of angiogenesis in diabetic rats with cerebral ischemic injury
HAN Jiang-quan1, LU Jun-jiang1,3, XIANG Can-hui2, LIU Cheng-ling1, WANG Zheng-yuan1, LIU Ling1, CHEN Ling1, FAN Ya-dan1
1. Department of Neurology, The Fifth Affiliated Hospital, Zunyi Medical College, Zhuhai 519100, China;
2. Experimental Center of Zhuhai Campus, Zunyi Medical College, Zhuhai 519100, China;
3. Special Medical Center of Zhujiang Hospital Affiliated to Southern Medical University, Guangzhou 510000, China
AIM: To evaluate the effect of microRNA-155(miRNA-155) on the regulation of angiogenesis in diabetic rats with cerebral ischemic injury. METHODS: Adult male Sprague-Dawley rats were randomly divided into 5 groups:sham group, cerebral ischemia group, diabetic cerebral ischemia group, diabetic cerebral ischemia+miRNA-155 inhibitors group and diabetic cerebral ischemia+scramble group. Diabetes model was made by injection of streptozocin and permanent cerebral ischemic model was developed by suture-occluded method. The scores of neurological deficit and infarct volume were estimated at 24 h after cerebral ischemia. miRNA-155 level was detected by real-time polymerase chain reaction. The expression of platelet endothelial cell adhesion molecule-1(PECAM-1/CD31) and vascular endothelial growth factor(VEGF) was detected by Western blotting. RESULTS: miRNA-155 inhibitor significantly reduced miRNA-155 levels in the ischemic cortex(P<0.05), improved the scores of neurological deficit, reduced infarction size and upregulated the levels of CD31 and VEGF(P<0.05). CONCLUSION: miRNA-155 has a critical role in the regulation of angiogenesis in diabetic rats with cerebral ischemia. Down-regulation of miRNA-155 using miRNA-155 inhibitor attenuates brain infarct injury in diabetic rats.
韩江全, 卢俊江, 向灿辉, 刘承灵, 王正远, 刘玲, 陈玲, 范娅丹. MicroRNA-155对糖尿病大鼠脑缺血损伤血管再生的调控[J]. 中国病理生理杂志, 2015, 31(2): 354-358.
HAN Jiang-quan, LU Jun-jiang, XIANG Can-hui, LIU Cheng-ling, WANG Zheng-yuan, LIU Ling, CHEN Ling, FAN Ya-dan. Effect of microRNA-155 on regulation of angiogenesis in diabetic rats with cerebral ischemic injury. Chin J Pathophysiol, 2015, 31(2): 354-358.
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