AIM: To determine the effects of glutamine(Gln) pretreatment on occludin protein in the rats with intestinal ischemia-reperfusion(I/R) injury. METHODS: Male Wistar rats(n=30) were randomly divided into 3 groups(n=10):sham group, I/R group and Gln pretreatment group. The rats in Gln pretreatment group were pretreated with Gln at dose of 1 g·kg-1·d-1 by orogastric route for 7 d, and those in the other 2 groups were pretreated with the same volume of normal saline. Intestinal I/R was induced by 30-min occlusion of the superior mesenteric artery followed by 24 h of reperfusion. After the operation, the levels of IL-10, IL-2, TNF-α, SOD and MDA were measured. The occludin protein was determined by the methods of immunohistochemistry and Western blotting. RESULTS: The occludin protein level in I/R group was significantly lower than that in sham group and Gln group(P<0.05). The levels of MDA and TNF-α in I/R group were significantly higher than those in sham group and Gln group(P<0.05). The levels of SOD, IL-10 and IL-2 in I/R group were significantly lower than those in sham group and Gln group(P<0.05). CONCLUSION: Glutamine has a protective effect on occludin protein in intestinal ischemia-reperfusion injury. The mechanism may be rela-ted to oxidative stress response and inflammatory inhibition.
王爱丽, 牛琼, 史宁, 贾兴芳, 刘成霞. 谷氨酰胺对大鼠肠缺血再灌注损伤后闭合蛋白的保护作用[J]. 中国病理生理杂志, 2015, 31(2): 364-368.
WANG Ai-li, NIU Qiong, SHI Ning, JIA Xing-fang, LIU Cheng-xia. Protective effects of glutamine pretreatment on occludin protein in rats with intestinal ischemia-reperfusion injury. Chin J Pathophysiol, 2015, 31(2): 364-368.
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