Effects of RNA interference of FABP5 on subcutaneous tumor of human hepatocellular carcinoma cell line HepG 2 transplanted in nude mice
ZHOU Ling-li1,2, CAO Ji1, LI Wei1,2, LUO Wang1,2, YANG Xiang-di1,2, YANG Chun1, LUO Cheng-piao1, TANG Yan-ping1, LI Yuan1
1. Research Department, Guangxi Cancer Institute of Guangxi Zhuang Autonomous Region, Nanning 530021, China;
2. Graduate School of Guangxi Medical University, Nanning 530021, China
AIM: To investigate the effect of recombinant lentiviral vector for RNA interference (RNAi) on the expression of fatty acid-binding protein 5 (FABP5) gene in hepatocellular carcinoma HepG2 cells and tumor formation in nude mice.METHODS: RNAi lentiviral vector was used in the experiment. Human hepatocellular carcinoma HepG2 cells were divided into 3 groups:the HepG2 cells in experimental group were transfected with the recombinant lentivirirus vector LV-shRNA-FABP5, the cells in negative control group were transfected with a control lentiviral vector LV-shRNA-NC, and the cells in normal control group were without any treatment. The nude mice were randomly divided into 3 groups. The growth of the transplanted tumor cells in the nude mice was observed. The tumor growth curve, volume and weight were determined 4 weeks after the cell inoculation. The expression of FABP5 was detected by real-time PCR, Western blot and immunohistochemical staining.RESULTS: Transfection of the lentiviral vector FABP5-shRNA obviously reduced FABP5 expression in the HepG2 cells. Tumor formation was all positive in the 3 groups of the nude mice inoculated with the tumor cells. Compared with normal control group and negative control group, the tumor growth slowed significantly in experimental group with smaller volume and weight. FABP5 expression in the transplanted tumor tissues was significantly down-regulated at mRNA and protein levels in experimental group as compared with normal control group and negative control group.CONCLUSION: RNAi-induced down-regulation of FABP5 effectively inhibits the growth of transplanted hepatocellular carcinoma, suggesting that FABP5 gene may be an effective target for gene therapy in treating liver cancer.
周玲丽, 曹骥, 李薇, 罗旺, 杨香娣, 杨春, 骆成飘, 唐艳萍, 李瑗. RNA干扰FABP5基因对人肝癌HepG2细胞裸鼠移植瘤生长的影响[J]. 中国病理生理杂志, 2015, 31(4): 603-608.
ZHOU Ling-li, CAO Ji, LI Wei, LUO Wang, YANG Xiang-di, YANG Chun, LUO Cheng-piao, TANG Yan-ping, LI Yuan. Effects of RNA interference of FABP5 on subcutaneous tumor of human hepatocellular carcinoma cell line HepG 2 transplanted in nude mice. Chin J Pathophysiol, 2015, 31(4): 603-608.
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