Synergistic effect of hypertension and type 2 diabetes on cardiac dysfunction and myocardial remodeling in mice
HUANG Mei-song1, LIU Peng2, CHEN Li-bing1, HU Yang-qian2
1. Department of Endocrinology, Shiyan Hospital of Integrated Traditional and Western Medicine, Shiyan 442011, China;
2. Department of Gastroenterology, The Affiliated Dongfeng Hospital, Hubei University of Medicine, Shiyan 442008, China
AIM: To investigate whether the effect of hypertension combined with diabetic can lead to cardiac dysfunction and myocardial remodeling in mice. METHODS: The diabetic mice and non-diabetic mice of 14 weeks old were administered with PBS or angiotensin II (Ang II) for 4 weeks to induce mild hypertension. The left ventricular (LV) function was assessed by echocardiography and dobutamine stress test. The LV tissues were subjected to HE staining to assess cardiac hypertrophy. The phospharylated adenosine monophosphate-activated protein kinase (p-AMPK) levels in the LV tissues were determined by Western blotting. RESULTS: Compared with control group, diabetic mice (DM group) neither displayed marked cardiac dysfunction nor myocardial remodeling. Ang II treatment did not affect body weight and glucose level, but the blood pressure was increased in the diabetic and control mice. Ang II-induced LV hypertrophy in diabetic mice was significantly higher than that in control mice as assessed by LV masses and cardiomyocyte sizes. Moreover, Ang II-treatment reduced LV fractional shortening and contractility in the diabetic mice, but not in the control mice. The p-AMPK levels were significantly reduced in Ang II group, DM group and DM+Ang II group. CONCLUSION: The cardiac function and cardiac structure of type 2 diabetic mice did not obviously change. Cardiac dysfunction and myocardial remodeling were easily induced in type 2 diabetic mice when hypertension happened, suggesting that hypertension is a critical factor of cardiac dysfunction and myocardial remodeling in type 2 diabetic mice.
黄美松, 刘鹏, 陈立兵, 胡阳黔. 高血压与2型糖尿病协同作用导致小鼠心功能障碍和心肌重塑[J]. 中国病理生理杂志, 2015, 31(6): 988-994.
HUANG Mei-song, LIU Peng, CHEN Li-bing, HU Yang-qian. Synergistic effect of hypertension and type 2 diabetes on cardiac dysfunction and myocardial remodeling in mice. Chin J Pathophysiol, 2015, 31(6): 988-994.
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